Recombinant DNA technology in therapeutic protein engineering - lecture course

After completion of the course, the students will recognize and are able to describe the most important methods in recombinant DNA technology (RDT, "molecular cloning"). They will know how to compare the advantages and disadvantages of commonly used approaches such as Restriction-enzyme cloning, PCR-based cloning, and multifragment assembly. They will be able to regogize and describe the most important methods of modifying genetic information (mutagenesis), including CRISPR-based gene editing, and they will be able to select appropriate RDT methods to real-world problems.

• Lecture slides (available via Moodle)
• 2nd chapter of the reference textbook (Molecular biotechnology: principles and applications of recombinant DNA, Glick & Patten, 2017(Opens in a new tab))
• Key publications for Restriction Enzyme Cloning, PCR, Gibson and Golden Gate Assembly, Site-directed Mutagenesis, etc. on Moodle

• Tutorial videos for all most common cloning procedures (Moodle links)

• Completion of the pre- and post-lecture assignments
• Participation in the contact sessions (80% of the lectures)

The final grade (on a pass/fail scale) is based on a Moodle exam about recombinant DNA technology.

Lectures, pre- and post-lecture assignments (which can be reading assignments, watching a video, short written assigments, quizzes, etc.)

Primary target group is students of PROV and MPHARM without any prior explicit or extensive knowledge of RDT. The course is open to all other BSc, MSc, PhD and exchange students. The course is optional for all target groups, and there is no limit on the number of participants.

3. teaching period

The course is recommended to take in the 3rd or 4th year of studies.

English

Level 7